000 02014cam a2200325 i 4500
999 _c1905
_d1905
001 19408536
005 20190314085045.0
008 161215s2016 njua b 001 0 eng
010 _a 2016440109
020 _a9781118873656 (cloth)
040 _aB-IKIAM
041 _aENG
082 0 0 _a571.5/38
_bF885
100 1 _aFreshney, R. Ian,
245 1 0 _aCulture of animal cells :
_ba manual of basic technique and specialized applications /
_cR. Ian Freshney, Institute of Cancer Sciences, University of Glasgow ; reviewing editors, Amanda Capes-Davis, Children's Medical Research Institute, University of Sydney, Westmead, Australia, Carl Gregory, Institute for Regenerative Medicine, Texas A&M Health Science Center, Temple, TX, USA, Stefan Przyborski, School of Biological & Biomedical Science, Durham University, Durham, UK.
250 _aSeventh edition.
264 1 _aHoboken, New Jersey :
_bWiley Blackwell,
_c[2016]
300 _axxxviii, 684 pages, 32 unnumbered pages of plates :
_billustrations (some color) ;
_c29 cm
336 _atext
_btxt
_2rdacontent
337 _aunmediated
_bn
_2rdamedia
338 _avolume
_bnc
_2rdacarrier
505 _aBiology of cultured cella - Laboratory desing and layout - Equipment and materials - Culture Vassels and substrates - Serum-free media - Defined media and supplements - Preparation and sterilization - Suplementary material - Primary culture - Supplementary material - Subculture and cell lines - Authentication and validation - Microbial contamination - Clonign and selection - Differentiation - Theree-dimensional culture - Cell line characterization - Cryopreservation and banking - Cell sorting - Scale-up and automation - Quantitation - Suplementary material
650 0 _aTissue culture
650 0 _aCell culture
700 1 _aCapes-Davis, Amanda,
700 1 _aGregory, Carl,
700 1 _aPrzyborski, Stefan,
942 _2ddc
_aB-IKIAM
_b14-3-2019
_cBK
_zbv