Animal Cell Culture Techniques [
edited by Martin Clynes
- XIII, 618 p. 34 illus. : online resource
I General Methods -- 1 Cloning Animal Cells -- 2 Cell Culture and Diagnostic Virology -- 3 Screening for Mycoplasma Contamination in Animal Cell Cultures -- 4 Serum-Free Media -- 5 Screening Culture Media and Sera for Endotoxin Testing -- 6 Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry -- 7 The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus -- 8 Primary Culture -- II Cell proliferation and Death -- 9 Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes -- 10 Methods for Studying Phosphoinositide Metabolism in Cultured Cells -- 11 Analysis of Cell Cycle and Cell Death Mechanisms -- III In Vitro Models for Cell Differentiation -- 12 Assessing Release of Secretory Products from Individual Cells -- 13 The Granulocyte/Macrophage Colony-Forming Unit Assay -- 14 Investigations into Cell Differentiation Using Cells in Culture -- 15 Cell Culture Models for Mononuclear Phagocytes -- 16 The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses -- 17 Cell Culture Models for Polarized Epithelial Monolayers -- 18 Ion Channels and Cell Signaling in Cell Cultures -- 19 Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells -- 20 Culture Systems for Hepatocytes for Use in Toxicology and Differentiation Studies -- IV In Vitro Models for Toxicology and Pharmacology -- 21 Cellular Models for In Vitro Toxicity Testing -- 22 Miniaturized In Vitro Methods in Toxicity Testing -- 23 Investigation of Anti-tumor Drug Accumulation and Efflux in Cell Culture -- 24 Current Status of Genetically Engineered In Vitro Systems for Biotransformation -- V Industrial Application of Animal Cell Culture -- 25 Scale-Up of Animal Cell Cultures -- 26 Purification of Monoclonal Antibodies Produced by Animal Cells in Culture -- 27 Biosafety Assessment of Cultured Animal Cells Used for Biopharmaceutical Production -- 28 Cytokine Cell Culture Assays -- VI Genetic Manipulation and Analysis of Human and Animal Cells in Culture -- 29 Antisense Methods in Cell Culture -- 30 Transfection with Ribozymes to Investigate the Role of Specific Gene Expression -- 31 Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture -- 32 Analysis of Specific and Differentially Expressed mRNAs in Cell Culture -- 33 Human Gene Therapy: Review and Outlook
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture